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Extra info for 3D Histology Evaluation of Dermatologic Surgery
1007/978-1-4471-4438-0_3, © Springer-Verlag London 2013 27 28 Chapter 3. 3 cm2 or 22 mm in length High effort for large tumors excision step at another day or even later is easy to manage if the patient is hospitalized or lives in the surroundings. The final choice between cryo- and paraffin sectioning used in the 3D histology depends on the expertise and special training of the surgeon and his collaboration with a pathologist. It is also influenced by the quality of the respective health-care system and the costs involved in each case.
9d). Now, the strips and bottom are placed with their outside down in a plane (Fig. 9e). Using this procedure, H&E stained sections can be taken from the complete outside of the tumor specimen. Vertical Excision and Square Procedure, Two Possibilities of Orientation To facilitate the flattening of the tumor margins, some surgeons prefer the removal of the tumor in a square-shaped specimen. Here the tumor with a safety margin is excised by four cuts forming a square. The 12 o’clock orientation differs in the examples shown (Fig.
4c, d) and clamped into the microtome (Fig. 4e). 4 Preparation of paraffin sectioning flipping over the paraffin block, now showing the complete 3D outside of the specimen in a 2D plane as a result of flipping over the block. The 3 o’clock position of the tissue margins will now be at the 9 o’clock position, and the outside of the margins will be on the surface of the paraffin block. Paraffin Sectioning After the paraffin block is clamped into the microtome, the sectioning of the tissue can begin (Fig.